Strain - BW21038/pLD54

Strain - BW21038/pLD54Strain BW21038/pLD54

Strain BW21038/pLD54

CGSC#: 7890
Strain Designation
Designation Source Preference
BW21038/pLD54 Wanner B.L. 1
Sex(Hfr,F+,F-,or F'): F-

Designation	Source	Preference
BW21038/pLD54	Wanner B.L.	1

6 Mutations:

Mutation	Location	Certainty	Display
DE(codB-lacI)3	7.60	1	Δ(codB-lacI)3
phoA532(del)	8.64	1	ΔphoA532
uidA3(del)::pir+	36.48	1	ΔuidA3::pir⁺
recA1	60.80	1	recA1
DE(phnP-phnD)3330( phnC?)	92.93	1	Δ(phnP-phnD)3330( phnC?)
mini-Tn5Lac4(CamR,lacIQ)	1000.00	1	mini-Tn5Lac4(CamR,lacI^Q)

Comments:
- pLD54 is the same as pBW30 except for the deletion of PstI site in bla. The pBW30 host is pir-116, recA⁺, and does not have mini-Tn5Lac4.
Episome/Plasmid: pLD54
Plasmid Markers/Mutations:
bla-1(ApR,dPstI)
oriR6Kgamma
oriT RP4
oriRg
oriT$_R4$
bla
References:
- Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
Printable Strain Report