Strain - EP432

Strain - EP432Strain EP432

Strain EP432

CGSC#: 7974
Strain Designation
Designation Source Preference
EP432 Schuldiner S. 1
Sex(Hfr,F+,F-,or F'): F-

7 Mutations:

Mutation	Location	Certainty	Display
thr-1	0.01	1	thr-1
nhaA1(del)::kan	0.38	1	ΔnhaA1::kan
DE(lacY-lacZ)9998	7.77	1	Δ(lacY-lacZ)9998
nhaB3(del)::Cm	26.57	1	ΔnhaB3::Cm
rpsL-(strR)	74.84	1	rpsL-(strR)
melB-(ts,LiD)	93.57	1	melB-(ts,LiD)
zjd-2334::Tn10	93.70	1	zjd-2334::Tn10

Comments:
- This strain is sensitive to Na+ and should be grown on LBK medium(LB plus KCl (6.5 g/l) lacking NaCl).
References:
- Pinner, E., Y. Kotler, E. Padan, S. Schuldiner 1993. Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli. J.Biol.Chem. 268:1729-1734
Printable Strain Report