Strain - HT306

Strain - HT306Strain HT306

Strain HT306

Designation	Source	Preference
HT306	Tabor H.	1

16 Mutations:

Mutation	Location	Certainty	Display
thr-1	0.01	1	thr-1
araC14	1.52	1	araC14
speD98(del)	2.91	1	ΔspeD98
DE(gpt-proA)62	5.51	1	Δ(gpt-proA)62
lacY1	7.79	1	lacY1
glnX44(AS)	14.99	1	glnX44(AS)
galK2(Oc)	16.99	1	galK2(Oc)
LAM-	17.40	1	λ^-
DE(speB-speA)97	66.39	1	Δ(speB-speA)97
DE(speC-glcB)63	66.92	1	Δ(speC-glcB)63
rpsL25(strR)	74.84	1	rpsL25(strR)
xylA5	80.34	1	xylA5
mtl-1	81.26	1	mtl-1
thiE1	90.34	1	thiE1
ampCp-1	93.80	1	ampCp-1
cadA2	93.85	1	cadA2

Comments:
- 1.Demonstration of the Spe^- phenotype requires care. Polyamines may be present even in purified agar, on glassware not acid-washed, on some plasticware and in rust-inhibitors in autoclave steam.(pers.comm.,H.Tabor).
- 2.The strain does require spermidine for optimum growth, but it may be apparent only with growth curves performed in liquid media, using spermidine-starved cells as an inoculum (pers.comm.,B.Weiss),using precautions to avoid contaminating polyamines.
References:
- Tabor, H., E.W. Hafner, C.W. Tabor 1980. Construction of an Escherichia coli strain unable to synthesize putrescine, spermidine, or cadaverine: characterization of two genes controlling lysine decarboxylase. J.Bacteriol. 144:952-956
Printable Strain Report