Strain - NH5054

Strain - NH5054Strain NH5054

Strain NH5054

CGSC#: 5970
Strain Designation
Designation Source Preference
NH5054 Howard-Flanders P. 1
Sex(Hfr,F+,F-,or F'): F-
8 Mutations:
Mutation Location Certainty Display
glnX44(AS) 14.99 1 glnX44(AS)
LAM- 17.40 1 λ^-
uvrB5 17.52 1 uvrB5
sbcB15 44.85 1 sbcB15
recB21 63.60 1 recB21
endA1 66.57 1 endA1
thiE1 90.34 1 thiE1
deo-27 or deo-43 99.00 1 deo-27 or deo-43
Comments:
- deo-27 is deoB or deoC. This strain is φX-sensitive but plates φX poorly with turbid plaques. This is an E. coli K-12 x C hybrid.
References:
- Cassuto, E, J Mursalim, P Howard-Flanders 1978. Homology-dependent cutting in trans of DNA in extracts of Escherichia coli: an approach to the enzymology of genetic recombination. Proc. Natl. Acad. Sci. U.S.A. 75(2):620-4.
Printable Strain Report

Mutation	Location	Certainty	Display
glnX44(AS)	14.99	1	glnX44(AS)
LAM-	17.40	1	λ^-
uvrB5	17.52	1	uvrB5
sbcB15	44.85	1	sbcB15
recB21	63.60	1	recB21
endA1	66.57	1	endA1
thiE1	90.34	1	thiE1
deo-27 or deo-43	99.00	1	deo-27 or deo-43