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Strain JA200

• CGSC#: 6059
• Strain Designation

  Designation	Source	Preference
  JA200	Carbon J.	1

  
  
• Sex(Hfr,F+,F-,or F'): F+
• 13 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  araC14	1.52	1	araC14
  leuB6(Am)	1.74	1	leuB6(Am)
  lacY1	7.79	1	lacY1
  glnX44(AS)	14.99	1	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  galT22	17.01	1	galT22
  LAM-	17.40	1	λ-
  trpE63(del)	28.44	1	ΔtrpE63
  recA56	60.80	1	recA56
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • As a ColE1-based pLC host: Colicin E1 can be used for selection (e.g., 1 unit/ml medium of commercially obtained prep, or 1/100 to 1/1000 dilution of lysate from induction of ColE1-carrying strain).
  • HOST STRAIN FOR pLC PLASMID LIBRARY
  • This strain is not a derivative of strain C600.
• References:
  • Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
  
  

  Printable Strain Report