CGSC Strain#: 5164
Strain Designation: D23      Source of Strain: H.G. Boman
Sex: F- Chromosomal Markers: proA23, lac-28, tsx-81, trp-30, his-51, mreB11, rpsL173(strR), sloB1, ampCp-1
Strain Comments:

• ampCp-1-- is a promoter mutation; AmpC⁺ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
• ampCp-1 was formerly called ampA1
• strR = streptomycin resist.

References:

• Normark, S, HG Boman, E Matsson 1969. Mutant of Escherichia coli with anomalous cell division and ability to decrease episomally and chromosomally mediated resistance to ampicillin and several other antibiotics. J. Bacteriol. 97(3):1334-42.
• Normark, S 1969. Mutation in Escherichia coli K-12 mediating spherelike envelopes and changes tolerance to ultraviolet irradiation and some antibiotics. J. Bacteriol. 98(3):1274-7.
• Westling-Haggstrom, B., S. Normark 1975. Genetic and physiological analysis of an envB spherelike mutant of Escherichia coli K-12 and characterization of its transductants. J.Bacteriol. 123:75-82