CGSC Strain#: 5624
Strain Designation: MA-1      Source of Strain: Broekman & Hoekstra
Other Designations: PC1978
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, codA1, lacY1, tsx-95, glnX44(AS), λ^-, pyrF101, his-108, fabB3, thyA6, argG66, rpsL201(strR), ilvA634, thiE1, deoC1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• strR = streptomycin resist.

References:

• Broekman, J.H.F.F., W.P.M. Hoekstra 1973. Mapping of fabC, a locus for the biosynthesis of unsaturated fatty acids in Escherichia coli K12. Mol.Gen.Genet. 124:65-0
• Clark, D, JE Cronan 1977. Further mapping of several membrane lipid biosynthetic genes (fabC, fabB, gpsA, plsB) of Escherichia coli. J. Bacteriol. 132(2):549-54.