CGSC Strain#: 4296
Strain Designation: KL355      Source of Strain: M.C. Cappello
Other Designations: KG20,F-
Sex: F- Chromosomal Markers: fhuA2::IS2, proA44, lacY1, glnX44(AS), galK2(Oc), λ^-, hisG1, rfbC1, galP63, malT1(λ^R), xyl-7, mtlA2, ΔargH1, rplL9(L?), ampCp-1, purA44
Strain Comments:

• The parent of KG20 was PA526 and not PA256 as in reference below.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• ampCp-1-- is a promoter mutation; AmpC⁺ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
• ampCp-1 was formerly called ampA1
• fhuA2::IS2 was formerly called fhuA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor
• L? = assignment uncertain
• LamR = Lambda-resistant
• Oc = ochre(UAA) mutation

References:

• Eriksson-Grennberg, KG 1968. Resistance of Escherichia coli to penicillins. II. An improved mapping of the ampA gene. Genet. Res. 12(2):147-56.