CGSC Strain#: 4301
Strain Designation: KL716      Source of Strain: M.C. Cappello
Other Designations: KLF48/KL158
Sex: F' Chromosomal Markers: Δ(gpt-proA)62, lacY1, tsx-29, glnX44(AS), galK2(Oc), λ^-, aroD5, hisG4(Oc), recA1, xylA5, argE3(Oc), thiE1, str-148
Strain Comments:

• This strain must be grown on medium containing B₁, Pro, Arg, and lacking His, Try, Tyr, Phen.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References: