CGSC Strain#: 6715
Strain Designation: GR401      Source of Strain: I.R. Booth
Sex: F- Chromosomal Markers: [araD139]_B/r, Δ(argF-lac)169, λ^-, flhD5301, Δ(his-gnd)296, relA1, rpsL150(strR), cycA30::Tn10, deoC1
Strain Comments:

• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• flhD5301-- flagellar defect shown in Komeda and Iino
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• = tetracycline resistant
• strR = streptomycin resist.

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