CGSC Strain#: 6831
Strain Designation: PK122      Source of Strain: W.K. Maas
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ^-, trp-35, Rac-0, hisG4(Oc), rfbC1, lysP37, thyA20, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1, deo-72
Strain Comments:

• (Ref. below to lysP37.)
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• thyA20-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• deo-72-- is a deoC or deoB mutation
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• lysP37 was formerly called cadR37
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Popkin, P.S., W.K. Maas 1980. Escherichia coli regulatory mutation affecting lysine transport and lysine decarboxylase. J.Bacteriol. 141:485-492