CGSC Strain#: 6847
Strain Designation: MFT84      Source of Strain: Ricard & Hirota
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), λ^-, trp-1, hisG1, rfbC1, nrdB84(ts), thyA719, galP63, malT1(λ^R), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1, deo-73
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• nrdB84(ts)-- is a temperature-sensitive, salt-rescuable mutant with fts-like pheno., hydroxyurea sensitive on low salt, that proved to be a mutation in distal region of B2 Nrd subunit, resulting in thermolability.
• thyA719-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• deo-73-- is either deoB or deoC.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• nrdB84(ts) was formerly called ftsB84
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• L? = assignment uncertain
• LamR = Lambda-resistant
• ts = Temperature Sensitive

References:

• Ricard, M., Y. Hirota 1973. Process of cellular division in Escherichia coli: physiology study on thermosensitive mutants defective in cell division. J.Bacteriol. 116:314-0
• Nishimura, Y., Y. Takeda, A. Nishimura, H. Suzuki, M. Inouye, Y. Hirota 1977. Synthetic ColE1 plasmids carrying genes for cell division in Escherichia coli. Plasmid 1:67-77