CGSC Strain#: 6860
Strain Designation: GM10     
Source of Strain: J.R. Walker
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA2::IS2,
pro-33,
lacY1,
argU110(ts)
,
glnX44(AS)
,
gal-6,
λ-,
hisG1,
rfbC1,
galP63,
xyl-7,
mtlA2,
ΔargH1,
rplL9(L?)
,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- argU110(ts)-- Pleiotropic properties of argU110(ts) mutant result fr. reduced intracell/ levels of this minor tRNA(Arg)--ca. 1/10 WT level, further reduced at 43C. reduction prob.due to defective interx.w/ Tu. ArgS
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- argU110(ts) was formerly called argU10
- argU110(ts) was formerly called dnaY10
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- L? = assignment uncertain
- ts = Temperature Sensitive
References: - Henson, J.M., H. Chu, C.I. Irwin, J.R. Walker 1979. Isolation and characterization of dnaX and dnaY temperature sensitive mutants of Escherichia coli. Genetics 92:1041-1059