CGSC Strain#: 6882
Strain Designation: SK3451     
Source of Strain: S.R. Kushner
Sex: F-
Chromosomal Markers: Δ(lacY-lacZ)286,
tsx-3,
glnX44(AS)
,
λ-,
lacZ95=phi80dIIlacZ9,
hisG4(Oc)
,
rpsL281(strR)
,
xyl-7,
mtl-1,
uvrD282::Tn5,
metE46,
ΔargH1Strain Comments: - Δ(lacY-lacZ)286-- is a deletion of part of lacY and part of lacZ which has been called lacMS286.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- lacZ95=phi80dIIlacZ9-- is an intragenic deletion of lacZ which does not overlap DE286, carried on φ80. (Konrad, 1977, J.Bacteriol.130:167)
- Δ(lacY-lacZ)286 was formerly called lacMS286
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- lacZ95=phi80dIIlacZ9 was formerly called lacBK1
- xyl-7 was formerly called xylA7
- = kanamycin resistant
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Maples, V.F., S.R. Kushner 1982. DNA repair in Escherichia coli: identification of the uvrD gene product. Proc.Natl.Acad.Sci.USA 79:5616-5620