CGSC Strain#: 6911
Strain Designation: CS1562     
Source of Strain: C.A. Schnaitman
Sex: F-
Chromosomal Markers: λ-,
rpoS396(Am)
,
tolC6::mini-Tn10,
rph-1Strain Comments: - tolC6::mini-Tn10-- Do not grow the strain on MacConkey or EMB or other media with dyes or detergents which act as counterselective agents and select for revertants.
- tolC6::mini-Tn10-- : This allele can be lost in the absence of tetracycline. The strain must be monitored frequently.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = tetracycline resistant
- Am = amber(UAG) mutation
References: - Austin, E.A., J.F. Graves, L.A. Hite, C. Parker, C.A. Schnaitman 1990. Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: insertion mutagenesis of the rfa locus. J.Bacteriol. 172:5312-5325