CGSC Strain#: 7044
Strain Designation: BW372     
Source of Strain: B. Weiss
Other Designations: RPC37
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
qsr'-0,
glnX44(AS)
,
galK2(Oc)
,
λ-,
Rac-0,
nth-1::kan,ble,
hisG4(Oc)
,
rfbC1,
mgl-51,
rpsL31(strR)
,
kdgK51,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- nth-1::kan,ble-- = insertion of cloned segment of Tn 5 which confers resistance to kanamycin and bleomycin.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- = kanamycin resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Cunningham, R.P., B. Weiss 1985. Endonuclease III (nth) mutants of Escherichia coli. Proc.Natl.Acad.Sci.USA 82:474-478