BW535      Source of Strain:
F- Chromosomal Markers: thr-1
, thiE1Strain Comments:
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- nth-1::kan,ble-- = insertion of cloned segment of Tn 5 which confers resistance to kanamycin and bleomycin.
- Δ(xthA-pncA)90-- Deleted region includes at least xthA,ydjXYZ,ynjABCDEFGH,gdhA,ynjI,topB,selD,ydjA, sppA,ansA and pncA
- nfo-1::kan-- insert of cloned segment of Tn903 which confers resistance to kanamycin.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- = kanamycin resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- Cunningham, R.P., S.M. Saporito, S.G. Spitzer, B. Weiss 1986. Endonuclease IV (nfo) mutant of Escherichia coli. J.Bacteriol. 168:1120-1127