CGSC Strain#: 4375
Strain Designation: KL405     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
lacZ36,
proC32,
hisF860(Stable)
,
thyA54,
rpsE2115(SpcR)
,
rpsL109(strR)
,
malA38,
xylA5,
mtl-1,
polA12(ts)
,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- thyA54-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- malA38-- : In Χ65 a Mal+ phenotype may have resulted from suppression of malA1, but in the absence of evidence, we show the mutations as malA38 and sup-78.
- polA12(ts)-- : sensitive to temperature and to low salt; altered pH optimum. Normal Exo levels. Totally defective in nick translation at 42C. recA56 polA12 are ts for growth.
- proC32 was formerly called proC65
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- SpcR = spectinomycin resistant
- Stable = stable, non-reverting
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Lloyd, R.G., K.B. Low, G.N. Godson, E.A. Birge 1974. Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive RecA- phenotype. J.Bacteriol. 120:407-415