CGSC Strain#: 4445
Strain Designation: LCB498     
Source of Strain: Cruezet-Sigal
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA2::IS2,
pro-33,
lacY1,
purE43,
glnX44(AS)
,
gal-6,
λ-,
hisG1,
rfbC1,
galP63,
Δ(gltB-gltF)500,
malT1(λ
R)
,
glgA1,
xyl-7,
mtlA2,
ΔargH1,
rplL9(L?)
,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- Δ(gltB-gltF)500-- :Glt- phenotype in gltB mutant requires presence of a gdhA mutation as well. 0.2% aspartate is a better N-source than glutamate because of uptake(Pahel et al).
- Δ(gltB-gltF)500 was formerly called aspB24
- Δ(gltB-gltF)500 was formerly called aspB21
- Δ(gltB-gltF)500 was formerly called gltB31
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- L? = assignment uncertain
- LamR = Lambda-resistant
References: - Sigal, N., J. Puig 1968. Compt.Rend.Acad.Sci.Paris 267:1223-0
- Cattaneo, J, M Damotte, N Sigal, F Sanchez-Medina, J Puig 1969. Genetic studies of Escherichia coli K 12 mutants with alterations in glycogenesis and properties of an altered adenosine diphosphate glucose pyrophosphorylase. Biochem. Biophys. Res. Commun. 34(5):694-701.