CGSC Strain#: 8009
Strain Designation: EDCM142      Source of Strain: M. Masters
Other Designations: TOP10/pTOF70
Sex: F- Episome/Plasmid: pTOF70
   Plasmid Markers/Mutations: FLCP3(CmR)
   Plasmid Comment: : FRT-{\it lac-cam-lacZp}-FRT cassette insertion
Chromosomal Markers: Δ(araA-leu)7697, [araD139]_B/r, Δ(codB-lacI)3, φ80dlacZ58(M15), galK0, mcrA0, galU-, recA1, endA1, nupG-, rpsL-(strR), Δ(mcrC-mrr)715
Strain Comments:

• Host strain TOP10.
• Δ(araA-leu)7697-- Shown by Durfee et al. to be a deletion of the first 295 codons of hepA to the first 82 codons of fruR.
• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• Δ(mcrC-mrr)715-- Deletes mcrBC-hsdSMR-mrr
• Δ(araA-leu)7697 was formerly called Δ(hepA-fruR)7697
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• = good miniprep dsDNA quality
• strR = streptomycin resist.
• CmR = chloramphenicol resistant

References:

• Merlin, C., S. McAteer, M. Masters 2002. Tools for characterization of Escherichia coli genes of unknown function. J.Bacteriol. 184:4573-4581