CGSC Strain#: 249
Strain Designation: PA214S
R,T6
R     
Source of Strain: F. Jacob
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA2::IS2,
lacY1,
tsx-2,
glnX44(AS)
,
gal-6,
λ-,
hisG1,
rfbC1,
galP63,
rpsL16(strR)
,
malT1(λ
R)
,
xyl-7,
mtlA2,
ppc-2,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- ppc-2 was formerly called glu-1
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- strR = streptomycin resist.
References: - Glansdorff, N. 1965. Topography of cotransducible arginine mutations in Escherichia coli K-12. Genetics 51:167-179