CGSC Strain#: 12116
Strain Designation: BW14012      Source of Strain: B.L. Wanner
Sex: F- Chromosomal Markers: Δ(codB-lacI)3, ΔphoA532, Δ(phnJ-mel)524(Tn5-1/132), [phn_B]
Strain Comments:

• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• [phn_B]-- functional phn locus from E. coli B
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• = tetracycline resistant
• Tn5-1/132 = See Metcalf,Wanner JB 173:590

References:

• Metcalf, W.W., B.L. Wanner 1991. Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi. J.Bacteriol. 173:587-600