CGSC Strain#: 6622
Strain Designation: JE5784     
Source of Strain: H. Suzuki
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
secA206(aziR)
,
mrcB704,
fhuA2::IS2,
tsx-67,
purE42,
glnX44(AS)
,
λ-,
trpE38,
his-208,
argG77,
rpsL109(strR)
,
xylA5,
mtl-1,
ilvA681,
thiE1,
metA160Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- mrcB704-- has been called ponB704. The gene encodes peptidoglycan synthetase, PBP-1Bs.
- purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal- phenotype, sup-78,near his.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- aziR = azide resistance
- strR = streptomycin resist.
References: - Suzuki, H, Y Nishimura, Y Hirota 1978. On the process of cellular division in Escherichia coli: a series of mutants of E. coli altered in the penicillin-binding proteins. Proc. Natl. Acad. Sci. U.S.A. 75(2):664-8.