CGSC Strain#: 8012
Strain Designation: EDCM71      Source of Strain: M. Masters
Other Designations: DH5α/pTOF25
Sex: F- Episome/Plasmid: pTOF25
   Plasmid Markers/Mutations: repA101(ts), kan, cat, sacB
   Plasmid Comment: Construction replacement vector derived from pKO3 and pUC4K. Cloning into pTOF25 using salI-smaI results in screenable loss of kan marker.
Chromosomal Markers: Δ(argF-lac)169, φ80dlacZ58(M15), glnX44(AS), λ^-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1, thiE1, hsdR17
Strain Comments:

• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• gyrA96(NalR)-- resistant to 200ug/ml nalidixic acid
• gyrA96(NalR)-- GAC to AAC at codon 87
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• gyrA96(NalR) was formerly called
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.
• = kanamycin resistant
• ts = Temperature Sensitive

References:

• Merlin, C., S. McAteer, M. Masters 2002. Tools for characterization of Escherichia coli genes of unknown function. J.Bacteriol. 184:4573-4581