CGSC Strain#: 3294
Strain Designation: AB3294      Source of Strain: A.J. Pittard
Other Designations: PC 1550
Sex: F- Chromosomal Markers: Δ(gpt-proA)62, lacY1, tsx-358, glnX44(AS), galK2(Oc), pabB3, hisG4(Oc), rpsL704(strR), xylA5, mtl-1, ilvC7, argE3(Oc), thiE1, tfr-3, fer-351
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• fer-351-- is a mutation affecting fertility.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Huang, M, J Pittard 1967. Genetic analysis of mutant strains of Escherichia coli requiring p-aminobenzoic acid for growth. J. Bacteriol. 93(6):1938-42.