CGSC Strain#: 4538
Strain Designation: AT3141     
Source of Strain: A.L. Taylor
Sex: Hfr
Point of Origin#: 12
Map Position: 68.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: thr-1,
leuB6(Am)
,
proA30,
lacZ4,
glnX44(AS)
,
λ-,
rpsL8,
argD37,
thiE1,
mu+Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- proA30-- may be due to integration of phage mu in the proA locus.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- argD37-- All argD mutants are quite leaky for Arg requirement. Best detected by suppression of pro- to Pro+.
- argD37-- 1.is leaky; We are told that this allele behaves better when transduced into another background. Suppresses proA30 to Pro+.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
References: - Taylor, A.L. 1970. Current linkage map of Escherichia coli. Bacteriol.Rev. 34:155-175