CGSC Strain#: 4550
Strain Designation: AT2036      Source of Strain: A.L. Taylor
Sex: F- Chromosomal Markers: Δ(gpt-proA)62, lacY1, tsx-29, glnX44(AS), suc-7::Mu, galK2(Oc), λ^-, hisG4(Oc), rpsL123(strR), xylA5, mtl-1, argE3(Oc), thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Taylor, A.L., M.S. Thoman 1964. The genetic map of Escherichia coli K-12. Genetics 50:659-663
• Taylor, A.L., C.D. Trotter 1967. Revised linkage map of Escherichia coli. Bacteriol.Rev. 31:332-353
• Herbert, AA, JR Guest 1968. Biochemical and genetic studies with lysine+methionine mutants of Escherichia coli: lipoic acid and alpha-ketoglutarate dehydrogenase-less mutants. J. Gen. Microbiol. 53(3):363-81.