CGSC Strain#: 4551
Strain Designation: AT1036      Source of Strain: A.L. Taylor
Sex: F- Chromosomal Markers: dapD4, Δ(gpt-proA)62, lacY1, glnX44(AS), λ^-, rfbC1, mgl-51, lysA27::Mu, rpsL20(strR), xylA5, mtl-1, thiE1
Strain Comments:

• The strain must be grown on medium fortified with 40-50 ug/ml diaminopimelic acid. It grows slowly. Excess lysine is inhibitory. In minimal medium use only 10 ug/ml of L-lysine.
• dapD4-- Strains with this mutation require 100ug/ml diaminopimelic acid even when grown on LB
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• lysA27::Mu-- mutation is due to the integration of phage Mu at the lysA locus.
• Δ(gpt-proA)62 was formerly called proA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• strR = streptomycin resist.

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