CGSC Strain#: 4553
Strain Designation: AT3196      Source of Strain: A.L. Taylor
Other Designations: BD1735
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, e14-, pdxH15(Am), relA1, spoT1, thiE1
Strain Comments:

• The mutant specifically requires pyridoxal, which must be added to both minimal and rich media for optimum growth (2 x 10^-6 M).
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation

References:

• Taylor, A.L. 1972. In Miller,Expts.in Molec.Genet. CSHL:451-0