CGSC Strain#: 5566
Strain Designation: JF618      Source of Strain: J.D. Friesen
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, glnX44(AS), galK2(Oc), λ^-, hisG4(Oc), cdd-6, pyrG51, rpsL31(strR), xylA5, mtl-1, pyrE60, argE3(Oc), thiE1
Strain Comments:

• Note that this strain requires added cytidine, even in rich media.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Friesen, JD, J Parker, RJ Watson, NP Fill, S Pedersen, FS Pedersen 1976. Isolation of a lambda transducing bacteriophage carrying the relA gene of Escherichia coli. J. Bacteriol. 127(2):917-22.