CGSC Strain#: 5730
Strain Designation: UTH6406      Source of Strain: T.S. Matney
Sex: F- Chromosomal Markers: leuB6(Am), fhuA2::IS2, lacY1, gal-6, λ^-, hisG1, argG6, mdh-10, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1, tyr-8
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• mdh-10-- It has been noted that mdh mutants in general are leaky and are distinguished from wild-type alleles by small colony size at 24-36 hours on medium with malate (autoclaved and at pH 6.0).
• fhuA2::IS2 was formerly called fhuA2
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• LamR = Lambda-resistant

References:

• Shaw, CR, JN Baptist, DA Wright, TS Matney 1973. Isolation of induced mutations in E. coli affecting the electrophoretic mobility of enzymes. Mutat. Res. 18(3):247-50.
• Heard, JT, MA Butler, JN Baptist, TS Matney 1975. Chromosomal location of mutations affecting the electrophoretic mobility of malate dehydrogenase in Escherichia coli K-12. J. Bacteriol. 122(1):329-31.