CGSC Strain#: 5800
Strain Designation: DG31      Source of Strain: D.H. Gelfand
Sex: F- Chromosomal Markers: Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ^-, Rac-0, sbcB15, hisG4(Oc), rfbC1, recB21, recC22, rpsL31(strR), kdgK51, xylA5, mtl-1, ilvE12, argE3(Oc), thiE1, hsdS14, hppT-29
Strain Comments:

• 1.Note on tyrB^- and aspC^- strains:
• 2.For growth of these strains, rich media should be supplemented with tyrosine and phenylalanine (50 ug/ml).
• 3.Minimal medium should be supplemented with asparagine, glutamic acid and glutamine (100 ug/ml), succinate (2.5 mg/ml), malate (2.5 mg/ml) and a-ketoglutarate (1 mg/ml).
• 4.For a discussion of the problems encountered in using these markers in transduction studies, see Gelfand & Rudo 1977 J. Bacteriol. 130:441.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• hppT-29-- : perhaps pHPP transport. spontaneous mutation of DG14 that allowed utilization of p-hydroxyphenylpyruvate to satisfy the Tyr requirement of tyrA16. Unmapped
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Gelfand, DH, RA Steinberg 1977. Escherichia coli mutants deficient in the aspartate and aromatic amino acid aminotransferases. J. Bacteriol. 130(1):429-40.
• Gelfand, D.H., N. Rudo 1977. Mapping of the aspartate and aromatic amino acid amino-transferase genes tyrB and aspC. J.Bacteriol. 130:441-444