CGSC Strain#: 6425
Strain Designation: PLK831     
Source of Strain: P. Kuempel
Sex: F-
Chromosomal Markers: gal-25,
λ-,
ΔtrpE63,
pyrF287,
fnr-1,
IN(rrnD-rrnE)1,
rpsL195(strR)
,
iclR7(Const)
,
trpR72(Am)
Strain Comments: - pyrF287-- marker comes from Hfr 3000 YA287 and is described as a stable allele.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- iclR7(Const)-- renders this strain constitutive for isocitrate lyase.
- fnr-1 was formerly called nirR1
- ΔtrpE63 was formerly called DE(trpE5)
- Am = amber(UAG) mutation
- Const = constitutive
- strR = streptomycin resist.
References: - Sternglanz, R., S. DiNardo, K.A. Voelkel, Y. Nishimura, Y. Hirota, K. Becherer, L. Zumstein, J.C. Wang 1981. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and translation. Proc.Natl.Acad.Sci.USA 78:2747-2751
- Bitner, R.M., P. Kuempel 1982. P1 transduction mapping of the trg locus in rac+ and rac strains of Escherichia coli K-12. J.Bacteriol. 149:529-533