CGSC Strain#: 12348
Strain Designation: SQ171      Source of Strain: Quan
Sex: F- Episome/Plasmid: ptRNA67
   Plasmid Markers/Mutations: ori_p15A, aadA(strR,SpcR), trpT , thrV , ileU , aspT , alaU , gltT
   Plasmid Comment: This plasmid was made by inserting the gene encoding tRNAglu2 into ptRNA66
Episome/Plasmid: pKK3535
   Plasmid Markers/Mutations: bla(ApR), ori_pMB1, rrsB , gltT , rrlB , rrfB
   Plasmid Comment: This plasmid contains the rrnB cluster cloned into the BamHI site of pBR322
Chromosomal Markers: Δ(rrsH-aspU)794(::FRT), λ^-, Δ(rrfG-rrsG)791(::FRT), Δ(rrfF-rrsD)793(::FRT), rph-1, Δ(rrsC-trpT)795(::FRT), Δ(rrsA-rrfA)792(::FRT), Δ(rrsB-rrfB)790(::FRT), Δ(rrsE-rrfE)789(::FRT)
Strain Comments:

• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• SpcR = spectinomycin resistant
• strR = streptomycin resist.
• ApR = ampicillin resistant

References:

• Quan, S, O Skovgaard, CL Squires 2010. Escherichia coli Ribosomal RNA deletion strain. unpublished results :0-0.