CGSC Strain#: 6451
Strain Designation: K603     
Source of Strain: N. Zinder
Sex: F+
Episome/Plasmid: F1-10(Tn10)
Plasmid Markers/Mutations: z--1::Tn10 Plasmid Comment: F1::Tn10, the integration of Tn10 in F1 to give F1-10 does not interfere with transfer functions.
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
ΔtrpE63,
xylA5,
mtl-1,
thiE1Strain Comments: - The integration of Tn 10 in F1 to give F1-10 does not interfere with transfer functions.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- ΔtrpE63 was formerly called DE(trpE5)
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- = tetracycline resistant
References: