CGSC Strain#: 12353
Strain Designation: C600/RK2      Source of Strain: F. Stahl
Sex: F- Episome/Plasmid: RK2
   Plasmid Markers/Mutations: bla(ApR), tetA, kan, kan , bla , tetA
   Plasmid Comment: Conjugative broad host-range plasmid
Chromosomal Markers: thr-1, leuB6(Am), fhuA21, cyn-101, lacY1, glnX44(AS), λ^-, e14-, rfbC1, glpR200(glp^c), thiE1
Strain Comments:

• It appears that some subcultures of C600 lost the e14 element and others carry it (e.g., C600, CGSC No.3004).
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• e14--- : Loss of the e14 element makes the strain McrA^-.
• cyn-101 was formerly called cyn-1
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• glp^c = constitutive expr.glp regulon
• = tetracycline resistant
• = kanamycin resistant
• ApR = ampicillin resistant

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