CGSC Strain#: 12352
Strain Designation: FS1290      Source of Strain: F. Stahl
Other Designations: FS594/RP4
Sex: F- Episome/Plasmid: RP4
   Plasmid Markers/Mutations: bla(ApR), kan, tetA, kan , bla , tetA
   Plasmid Comment: Broad host-range conjugative plasmid
Chromosomal Markers: lac-3350, galK2(Oc), galT22, λ^-, IN(rrnD-rrnE)1, rpsL179(strR), deoA21
Strain Comments:

• deoA21 was formerly called tpp-21.
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• galK2(Oc) was formerly called gal₂
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• = kanamycin resistant
• = tetracycline resistant
• ApR = ampicillin resistant

References:

• Fangman, WL, A Novick 1966. Mutant bacteria showing efficient utilization of thymidine. J. Bacteriol. 91(6):2390-1.