CGSC Strain#: 12408
Strain Designation: STL13779     
Source of Strain: S.T. Lovett
Sex: F-
Chromosomal Markers: lacZ4813,
mhpC281::Tn10,
λ-,
ΔmutS738::kan,
rph-1Strain Comments: - lacZ4813-- is a compound allele replacing GGC GGA GCC with GGG GGG -CC starting at base 1468 in the lacZ sequence. This results in two synonymous substitutions and a single basepair deletion of a G residue causing a frameshift mutation at base 1473.
- mhpC281::Tn10-- cotransduces 80% with lac.
- ΔmutS738::kan-- The primers used to make this knockout were AAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGATTCCGGGGATCCGTCGACC and CGATAGCAAAAGACTATCGGGAATTGTTATTACACCAGGCTCTTCAAGCGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- lacZ4813 was formerly called lacZ(+1G1473)
- mhpC281::Tn10 was formerly called zah281::Tn10
- = tetracycline resistant
- = kanamycin resistant
References: - Seier, T, DR Padgett, G Zilberberg, VA Sutera, N Toha, ST Lovett 2011. Insights into mutagenesis using Escherichia coli chromosomal lacZ strains that enable detection of a wide spectrum of mutational events. Genetics 188(2):247-62.