CGSC Strain#: 12395
Strain Designation: STL14025     
Source of Strain: S.T. Lovett
Sex: F-
Chromosomal Markers: lacZ4823,
mhpC281::Tn10,
λ-,
rph-1Strain Comments: - lacZ4823-- is a compound allele replacing ATACACT-----------TGCTGATGCGG with ATACACTTGCTGATGCGGTGCTGATGCGG starting at base 2486 in the lacZ sequence. This results in an 11 basepair duplication and a frameshift mutation.
- mhpC281::Tn10-- cotransduces 80% with lac.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- lacZ4823 was formerly called lacZ(+11)
- mhpC281::Tn10 was formerly called zah281::Tn10
- = tetracycline resistant
References: - Seier, T, DR Padgett, G Zilberberg, VA Sutera, N Toha, ST Lovett 2011. Insights into mutagenesis using Escherichia coli chromosomal lacZ strains that enable detection of a wide spectrum of mutational events. Genetics 188(2):247-62.