CGSC Strain#: 6465
Strain Designation: N2020      Source of Strain: D. Apirion
Sex: F- Chromosomal Markers: lacZ8(Am), trpA36, glyA34, argA52, rpsL999(strR), rnpA49(ts), ilvG866(Act)
Strain Comments:

• lacZ8(Am)-- From Miller's CSH manual: carries amber mutation at position 17 of lacZ (Kleina et al. 1990)
• rnpA49(ts)-- is a temperature-sensitive mutation.
• lacZ8(Am) was formerly called lac_ull8am
• Act = activates the Gene
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Apirion, D 1980. Genetic mapping and some characterization of the rnpA49 mutation of Escherichia coli that affects the RNA-processing enzyme ribonuclease P. Genetics 94(2):291-9.