CGSC Strain#: 12499
Strain Designation: N7105     
Source of Strain: R.G. Lloyd
Other Designations: AM1955
Sex: F-
Chromosomal Markers: λ-,
Δ(ruvB-ruvC)768(::apra),
rph-1Strain Comments: - For selection in transductions, use apramycin at a final concentration of no more than 30ug/ml in LB agar
- Δ(ruvB-ruvC)768(::apra)-- The deletion starts 39bp into ruvC and extends to 39bp from the end of ruvB. The deletion/insertion joints are as follows with chromosomal sequence in uppercase and apramycin cassette in lowercase: TCGCGCGTG/tcatgtgca..........tctgggcgg/TGGAATCAC.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- apra = Apramycin resistant
References: - Rudolph, CJ, AA Mahdi, AL Upton, RG Lloyd 2010. RecG protein and single-strand DNA exonucleases avoid cell lethality associated with PriA helicase activity in Escherichia coli. Genetics 186(2):473-92.