CGSC Strain#: 12489
Strain Designation: N6029     
Source of Strain: R.G. Lloyd
Other Designations: AM1651
Sex: F-
Chromosomal Markers: λ-,
Δ(ruvB-ruvA)799(::apra),
rph-1Strain Comments: - For selection in transductions, use apramycin at a final concentration of no more than 30ug/ml in LB agar
- Δ(ruvB-ruvA)799(::apra)-- The deletion starts 39bp into ruvA and extends to 39bp from the end of ruvB. The deletion/insertion joints are as follows with chromosomal sequence in uppercase and apramycin cassette in lowercase: ATTGAAAAA/tcatgtgca..........tctgggcgg/TGGAATCAC.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- apra = Apramycin resistant
References: - Grove, JI, L Harris, C Buckman, RG Lloyd 2008. DNA double strand break repair and crossing over mediated by RuvABC resolvase and RecG translocase. DNA Repair (Amst.) 7(9):1517-30.