CGSC Strain#: 12477
Strain Designation: N7568      Source of Strain: R.G. Lloyd
Other Designations: AM2037
Sex: F- Chromosomal Markers: λ^-, ΔrecA1908::cat, rph-1
Strain Comments:

• ΔrecA1908::cat-- The deletion starts 42bp into recA and extends to 45bp from the end of the gene. The deletion/insertion joints are as follows with chromosomal sequence in uppercase and cat cassette in lowercase: GCGGCAGCA/gtaagaggt..........atgatcttc/GTAGATGAT.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• cat = chloramphenicol acetyl transferase

References:

• Rudolph, CJ, AA Mahdi, AL Upton, RG Lloyd 2010. RecG protein and single-strand DNA exonucleases avoid cell lethality associated with PriA helicase activity in Escherichia coli. Genetics 186(2):473-92.