CGSC Strain#: 12427
Strain Designation: N4256      Source of Strain: R.G. Lloyd
Sex: F- Chromosomal Markers: λ^-, rph-1, recG263(::kan)
Strain Comments:

• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

References:

• McGlynn, P, RG Lloyd 2000. Modulation of RNA polymerase by (p)ppGpp reveals a RecG-dependent mechanism for replication fork progression. Cell 101(1):35-45.
• Jaktaji, RP, RG Lloyd 2003. PriA supports two distinct pathways for replication restart in UV-irradiated Escherichia coli cells. Mol. Microbiol. 47(4):1091-100.