CGSC Strain#: 6787
Strain Designation: BW7261     
Source of Strain: B.L. Wanner
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: leu-63::Tn10,
fhuA22,
Δ(argF-lac)169,
ompF627(T2R)
,
relA1,
spoT1Strain Comments: - Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
- Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- = tetracycline resistant
- T2R = T2 Resistant
References: - Wanner, B.L. 1986. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12. J.Mol.Biol. 191:39-58