CGSC Strain#: 12522
Strain Designation: DH5α/pBAD18-Kan      Source of Strain: J.R. Beckwith
Other Designations: DH5alpha/pBAD18-Kan
Other Designations: pBAD18-Kan
Sex: F- Episome/Plasmid: pBAD18-Kan
   Plasmid Markers/Mutations: ori_pMB1, araC , kan
   Plasmid Comment: Arabinose induced expression vector.
Chromosomal Markers: Δ(argF-lac)169, φ80dlacZ58(M15), glnX44(AS), λ^-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1, thiE1, hsdR17
Strain Comments:

• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• gyrA96(NalR)-- resistant to 200ug/ml nalidixic acid
• gyrA96(NalR)-- GAC to AAC at codon 87
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• gyrA96(NalR) was formerly called
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.

References:

• Guzman, LM, D Belin, MJ Carson, J Beckwith 1995. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177(14):4121-30.