CGSC Strain#: 4304
Strain Designation: KL164     
Source of Strain: M.C. Cappello
Sex: Hfr
Point of Origin#: 45
Map Position: 65.00
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ-,
e14-,
nalB4,
relA1,
thyA24,
spoT1,
thiE1,
deoB13Strain Comments: - e14--- : Loss of the e14 element makes the strain McrA-.
- nalB4-- : resistant to 6 ug/ml nalidixic acid in KL
- thyA24-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- thiE1 was formerly called thi-1
References: - Hane, M.W., T.H. Wood 1969. Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominant studies. J.Bacteriol. 99:238-241