CGSC Strain#: 5904
Strain Designation: PL1     
Source of Strain: P.S. Leboy
Sex: Hfr
Point of Origin#: 12
Map Position: 68.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: Δ(gpt-proA)62,
lacY1 or lacZ4,
glnX44(AS)
,
galK2(Oc)
,
λ-,
xylA5,
mtl-1,
thiE1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - LEBOY, PS, EC COX, JG FLAKS 1964. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI. Proc. Natl. Acad. Sci. U.S.A. 52:1367-74.