CGSC Strain#: 1132
Strain Designation: AB1132      Source of Strain: E.A. Adelberg
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, glnX44(AS), galK2(Oc), λ^-, hisG4(Oc), rpsL30(strR), xylA5, mtl-1, thiE1, met-33
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Zamenhof, PJ 1966. A genetic locus responsible for generalized high mutability in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 56(3):845-52.
• Williams, LS 1973. Control of arginine biosynthesis in Escherichia coli: role of arginyl-transfer ribonucleic acid synthetase in repression. J. Bacteriol. 113(3):1419-32.