CGSC Strain#: 3521
Strain Designation: AB3521     
Source of Strain: J.A. Wechsler
Other Designations: JW38
Sex: F'
Episome/Plasmid: F2016-1
Plasmid Markers/Mutations: ilvD188(Oc)
,
ilvE ,
ilvA Chromosomal Markers: araBAD-9,
fhuA1,
Δ(gpt-proA)62,
lacY1 or lacZ4,
tsx-3,
galK2(Oc)
,
trpE3(Oc)
,
hisG4(Oc)
,
relA1,
rpsL8 or rplL9(L?)
,
malT1(λ
R)
,
mtl-1,
ilvD188(Oc)
,
thiE1,
sup-48Strain Comments: - galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- sup-48-- weak amber suppressor.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- thiE1 was formerly called thi-1
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- Oc = ochre(UAA) mutation
References: - Wechsler, J.A. 1969. Antipolarity in the ilv operon of Escherichia coli K-12. J.Bacteriol. 98:1179-1194